Technology Platform

NIR-II Technology Principle

Biological tissue absorbs and scatters light differently across spectral bands, which determines the “readability” of every detection window. Conventional IVD platforms rely on visible light (400–780 nm) and the first near-infrared window (NIR-I, 700–900 nm), where strong tissue autofluorescence and optical scattering severely limit detection sensitivity and quantitative accuracy.

Compared to traditional visible light and NIR-I fluorescence, NIR-II region (1000–1700 nm) fluorescence exhibits deeper tissue penetration, lower background noise, and a higher signal-to-noise ratio. WWHS employs a unique encapsulation process to stably encapsulate hydrophobic NIR-II fluorescent dyes within polymer microspheres, ultimately preparing monodisperse functionalized nanospheres with a fluorescence quantum efficiency exceeding 20%. Infrared fluorescent POCT reagents developed based on these microspheres can be excited at 780 nm and detected at fluorescence levels above 1000 nm. Compared to traditional methods, NIR-II technology possesses stronger anti-interference capabilities, completely eliminating interference from the sample's own autofluorescence; simultaneously, leveraging the strong penetrating power of infrared light, it achieves 3D stereoscopic signal detection, reduces fluorescence scattering effects, and significantly improves detection sensitivity.

• Physical PrincipleNIR-II fluorescence imaging penetrates deep tissue and lowers background noise
• Complex SamplesStable and precise quantification even for hemolyzed, icteric, and lipemic samples
• Pioneering FoundationBuilt on the NIR-II fluorescence imaging principle pioneered by Professor Hongjie Dai's group